rabbit mab anti erg cell signaling technology cat  (Cell Signaling Technology Inc)

Journal: bioRxiv

Article Title: A FZD4/LRP5 agonist restores pericyte coverage and vascular integrity by increasing PDGFB signaling

doi: 10.64898/2026.03.13.711629

Figure Lengend Snippet: (A) Quantification of Pdgfb from total retina RNA was normalized to beta-actin , n=9-11 retinas from 9-11 mice per group. Average +/− SE is shown. *P < 0.05 by 1-way ANOVA with Tukey’s post hoc test. (B) Quantification of Pdgfb from total retina RNA was normalized to gapdh , n=5-7 retinas from 5-7 mice per group. Average +/− SE is shown. *P < 0.05 by 1-way ANOVA with Tukey’s post hoc test. ( C ) Images of anti-LEF1 stain of parental bEnd.3 cells and a stable population of bEnd.3 cells overexpressing LEF1, generated by lentiviral transduction and selection with puromycin. Scale bar 100 µm. ( D ) Quantification of Pdgfb from total RNA of bEnd.3 cells after treatment with vehicle or F4L5.13. 2 technical replicates per biological replicate were averaged, n=3 biological replicates. Average +/− SE is shown. *P < 0.05 by unpaired Student’s t-test. ( E ) Quantification of Pdgfb from total RNA of stable bEnd.3-LEF1 cells after treatment with vehicle or F4L5.13. 2 technical replicates per biological replicate were averaged, n=3 biological replicates. Average +/− SE is shown. *P < 0.05 by unpaired Student’s t-test.

Article Snippet: LEF1 was stained in PFA-fixed cells using rabbit anti-LEF1 (Cell Signaling, #2230S 1:100) and goat anti-rabbit Alexa Fluor-555 (Invitrogen, #A21428).

Techniques: Staining, Generated, Transduction, Selection